论文部分内容阅读
目的探讨不同佐剂对preS2/S乙型肝炎疫苗免疫原性的影响。方法将BALB/C小鼠随机分为6组:铝佐剂组(S+Al和S2/S+Al)、细胞因子佐剂组(S2/S+CK1、S2/S+CK2和S2/S+CK3)及阴性对照组(NG),用相应抗原腹腔免疫各组小鼠,1 mL/只,分别于免疫后2、4和6周采血,分离血清,ELISA法检测血清中抗-HBs抗体和preS2抗体水平;WST-1法检测淋巴细胞增殖情况。结果免疫后2周,铝佐剂组(S+Al和S2/S+Al)抗体水平较高,阳转率达100%,而细胞因子佐剂组抗-HBs抗体水平均较低,S2/S+CK2及S2/S+CK3组抗体阳转率为0,与NG组比较差异无统计学意义(P>0.05);免疫后4周,细胞因子佐剂组抗-HBs抗体水平升高明显,铝佐剂组S2/S+Al抗体水平显著高于S+Al(P<0.05);免疫后6周,细胞因子佐剂组抗体水平高于铝佐剂组,其中S2/S+CK1组抗体水平较高。免疫后2周,各组小鼠血清中抗-preS2抗体均达到较高水平,各组间差异均无统计学意义(P>0.05);免疫后4周和6周,抗体水平均有所下降,但下降不明显;铝佐剂组S+Al和S2/S+Al抗-preS2抗体水平差异无统计学意义(P>0.05)。免疫后4周,各组小鼠淋巴细胞刺激指数铝佐剂组(S+Al和S2/S+Al)低于细胞因子佐剂组,且差异有统计学意义(P<0.05),其中S2/S+CK1组淋巴细胞增殖水平较高。结论不同佐剂的preS2/S乙型肝炎疫苗均能诱导较高水平的抗-HBs和抗-preS2抗体,细胞因子佐剂组淋巴细胞增殖水平显著高于铝佐剂组,为新型乙型肝炎疫苗和治疗性乙型肝炎疫苗的研究提供了实验依据。
Objective To investigate the effect of different adjuvants on the immunogenicity of preS2 / S hepatitis B vaccine. Methods BALB / C mice were randomly divided into 6 groups: aluminum adjuvant (S + Al and S2 / S + Al), cytokine adjuvant (S2 / S + CK1, S2 / S + CK2 and S2 / S + CK3) and negative control group (NG). The mice in each group were immunized intraperitoneally with corresponding antigen (1 mL / mouse), and blood was collected at 2, 4 and 6 weeks after immunization. Serum anti-HBs antibodies And preS2 antibody levels; WST-1 method to detect lymphocyte proliferation. Results After 2 weeks of immunization, the antibody levels of Al + and S2 / S + Al were higher, the positive rate was 100%, but the level of anti-HBs antibody in the adjuvant was lower, The antibody positive rate of S + CK2 and S2 / S + CK3 group was 0, which showed no significant difference compared with NG group (P> 0.05). At 4 weeks after immunization, the level of anti-HBs antibody in cytokine adjuvant group was significantly increased , The level of S2 / S + Al antibody in aluminum adjuvant group was significantly higher than that in S + Al group (P <0.05). At 6 weeks after immunization, the antibody level of cytokine adjuvant group was higher than that of aluminum adjuvant group. Antibody level is higher. After 2 weeks of immunization, the serum anti-preS2 antibody in each group reached a higher level, and there was no significant difference among the groups (P> 0.05). After 4 weeks and 6 weeks of immunization, the antibody levels decreased , But the decrease was insignificant. The levels of S + Al and S2 / S + Al anti-preS2 antibodies in aluminum adjuvant group had no significant difference (P> 0.05). After 4 weeks of immunization, the lymphocyte stimulating index of mice in each group was lower than that of adjuvant group (P <0.05), and the difference was statistically significant (P <0.05) / S + CK1 group of lymphocyte proliferation higher. Conclusions PreS2 / S hepatitis B vaccine can induce higher levels of anti-HBs and anti-preS2 antibodies with different adjuvants. The cytokine adjuvant lymphocyte proliferation level was significantly higher than that of aluminum adjuvant group, which is a new type of hepatitis B Vaccines and therapeutic hepatitis B vaccine provides experimental evidence.