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在含苜蓿尺蠖核多角体病毒（ＡｃＮＰＶ）早期基因启动子的载体ｐＡｃＩＥ１中，插入人β－干扰囊（ＨｕＩＦＮ－β）基因，构建成转移载休ｐＩＥ１－ＩＢ；ｐＩＥ１－ＩＢ与含新霉素抗性基因（ＮＥＯ￣ｒ）的质粒ｐＩＥＩ－ＮＥＯ共转染秋粘虫细胞（Ｓｆ９细胞），使ＨｕＩＦＮ－β基因和ＮＥＯ￣ｒ基因整合到细胞基因组上产生转化细胞，含Ｇ４１８的培基分离新霉素抗性克隆，并增殖传代；转化细胞株传至５０代以上，不同传代水平细胞ＤＮＡ的点杂交证实ＨｕＩＦＮ－β基因已整合在细胞ＤＮＡ分子上，同时检测出干扰素（ＩＦＮ）的持续稳定表达，活性为５００—４０００ＩＵ／１０￣６细胞。抗天然ＨｕＩＦＮ－β抗体能完全中和所表达ＩＦＮ的抗病毒活性。
In the vector pAcIE1 containing the promoter of early gene of AcNPV, a human β-interferon (HuIFN-β) gene was inserted into pAcIE1 to construct a recombinant plasmid pIE1-IB. Plasmids pIEI-NEO containing the resistance gene (NEO-r) were cotransfected into fall armyworm cells (Sf9 cells) and the HuIFN-β and NEO-r genes were integrated into the cell genome to produce transformed cells. The culture medium containing G418 Neomycin-resistant clones, and passaged for passage. Transformed cell lines were passaged for more than 50 passages. Dot blotting of DNA at different passage levels confirmed that the HuIFN-β gene had been integrated into the cellular DNA molecules and that interferon (IFN) Sustained and stable expression, activity of 500-4000IU / 10 ~ 6 cells. Anti-native HuIFN-β antibodies completely neutralize the antiviral activity of IFN expressed.