论文部分内容阅读
目的:建立用高效液相色谱法同时测定代温灸膏中主要药效成分肉桂酸、桂皮醛、辣椒碱、二氢辣椒碱及6-姜酚的含量。方法:采用Alltima C18(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-0.1%磷酸,梯度洗脱,流速1 mL·min-1,检测波长280nm,柱温30℃。结果:肉桂酸、桂皮醛、辣椒碱、二氢辣椒碱、6-姜酚浓度分别在5.66～42.45,20.40～153.0,16.20～121.5,10.16～76.20,13.12～98.40μg.mL范围内与峰面积呈良好的线性关系。上述化合物的回收率和RSD分别为98.2%,1.4%;100.0%,1.0%;99.2%,1.1%;99.2%,1.2%;100.1%,1.0%。结论:该方法能准确、快速分析代温灸膏中5个主要成分,灵敏度高,重现性好,可用于控制代温灸膏的质量。
OBJECTIVE: To establish simultaneous determination of cinnamic acid, cinnamaldehyde, capsaicin, dihydrocapsaicin and 6-gingerol in modern moxibustion cream by high performance liquid chromatography. Methods: Alltima C18 (4.6 mm × 250 mm, 5 μm) column was used. The mobile phase consisted of acetonitrile-0.1% phosphoric acid with a gradient of 1 mL · min-1. The detection wavelength was 280 nm and the column temperature was 30 ℃. Results: The concentrations of cinnamic acid, cinnamaldehyde, capsaicin, dihydro capsaicin and 6-gingerol were respectively in the range of 5.66 ~ 42.45,20.40 ~ 153.0,16.20 ~ 121.5,10.16 ~ 76.20,13.12 ~ 98.40μg.mL and peak area A good linear relationship. The recovery and RSD of the above compounds were 98.2%, 1.4%; 100.0%, 1.0%; 99.2%, 1.1%; 99.2%, 1.2%; 100.1%, 1.0%, respectively. Conclusion: This method can accurately and quickly analyze the five main components of moxa moxibustion with high sensitivity and good reproducibility. It can be used to control the quality of moxibustion cream.