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为探讨低浓度多氯联苯(polychlorinated biphenyls,PCBs)商业混合物Aroclor1254对大鼠甲状腺结构及功能的影响,本文将12只雄性Wistar大鼠随机分为对照组和PCBs暴露组(Aroclor1254组),对照组腹腔注射Aroclor1254的溶剂玉米油,暴露组以2000μg·kg-1·d-1的剂量行腹腔注射Aroclor1254,每周注射5d,连续注射13周.采用HE染色、免疫组化及电镜技术观察PCBs对大鼠甲状腺组织结构的影响,酶联免疫吸附试验(ELISA)检测血清TSH、Free-T3、Free-T4水平,逆转录聚合酶链反应(RT-PCR)检测甲状腺球蛋白(Tg)及钠碘转运体(NIS)mRNA表达水平.研究结果发现,与对照组比较,暴露组大鼠甲状腺组织内单核巨噬细胞(ED-1+细胞)浸润增多,甲状腺微观结构受损,血清TSH水平显著升高,甲状腺组织TgmRNA及NISmRNA表达显著下调.研究结果表明,低浓度PCBs长期暴露可导致大鼠甲状腺结构破坏及功能损伤.
In order to investigate the effect of Aroclor 1254, a commercial mixture of low concentration PCBs, on the structure and function of thyroid in rats, 12 male Wistar rats were randomly divided into control group and exposed PCBs group (Aroclor 1254 group) Group A was injected intraperitoneally with solvent corn oil of Aroclor 1254. The exposed group received intraperitoneal injection of Aroclor 1254 at a dose of 2000 μg · kg-1 · d-1 for 5 days and then injected for 13 weeks continuously.Hematoxylin-eosin staining, immunohistochemistry and electron microscopy were used to observe the effects of PCBs On the thyroid tissue structure in rats. Serum levels of TSH, Free-T3 and Free-T4 were detected by enzyme-linked immunosorbent assay (ELISA), thyroglobulin (Tg) and sodium Iodine transporter (NIS) mRNA levels in the thyroid tissue of the exposed group were significantly higher than those of the control group (P <0.05). The results showed that the infiltration of monocyte-macrophage (ED-1 +) cells in the thyroid tissue of the exposed group was increased, the thyroid microstructure was damaged, Significantly increased thyroid tissue TgmRNA and NISmRNA expression was significantly reduced.The results show that long-term exposure to low concentrations of PCBs can lead to thyroid structural damage and functional damage in rats.