论文部分内容阅读
目的了解HBx和Bcclin1在原发性肝癌、癌旁组织的表达水平,及其在HCC的发病机制中作用及其相互关系。方法收集经病理确诊的HCC患者64例,患者术前术经任何抗肿瘤治疗,采用免疫组化、Western印迹及实时PCR方法检测HBx及Beclin 1在肝癌组织及其癌旁组织中的表达情况,并将实验结果与患者的临床资料结合进行统汁学分析。结果 53例患者的肝癌组织HBx蛋白表达量高于癌旁组织,差异有统计学意义(P<0.05);肝癌组织与癌旁组织中HBx与GAPDH相对灰度比值分别为1.54±0.13、0.62±0.07,肝癌组织表达量明显高于癌旁组织,差异有统计学意义(P<0.05)、肝癌组织与癌旁组织中HBx表达量分别为0.78±0.10、0.11±0.01,差异有统汁学意义(P<0.05)。51例患者肝癌组织Beclin1蛋白表达量高于癌旁组织,差异有统计学意义(P<0.05)。肝癌组织与癌旁组织中Bcclin1与GAPDH相对灰度比值分别为1.52±0.11、0.98±0.05,肝癌组织表达量叫显高于癌旁组织,差异有统计学意义(P<0.05)。肝癌组织与癌旁组织中Beclin1表达量分别为0.83±0.15、0.1 8±0.03,差异有统计学意义(P<0.05)。结论 HBx和Beclin1在HCC中高表达,两者相互作用且共同参与HCC的发生、发展过程,联合检测HBx和Beclin1对判断HCC的发生和转移有重要价值,是有潜力的肝癌治疗的候选靶基因。
Objective To investigate the expression of HBx and Bcclin1 in primary hepatocellular carcinoma and paracancerous tissues, and their role in the pathogenesis of HCC and their relationship. Methods 64 cases of HCC diagnosed by pathology were collected. Before operation, any anti-tumor therapy was performed. The expression of HBx and Beclin 1 in HCC tissues and their adjacent tissues was detected by immunohistochemistry, Western blot and real-time PCR. Combined the experimental results with the patient’s clinical data to analyze the system. Results The expression of HBx protein in HCC tissue of 53 patients was higher than that in adjacent tissues (P<0.05). The relative gray ratios of HBx to GAPDH in HCC and adjacent tissues were 1.54±0.13 and 0.62±, respectively. At 0.07, the expression of HCC was significantly higher than that of the adjacent tissues (P<0.05). The expression of HBx in HCC tissues and adjacent tissues was 0.78±0.10 and 0.11±0.01, respectively. (P<0.05). The expression of Beclin1 protein in hepatocellular carcinoma was higher in 51 patients than in adjacent tissues (P<0.05). The relative gray ratios of Bcclin1 and GAPDH in HCC tissues and adjacent tissues were 1.52±0.11, 0.98±0.05, respectively. The expression of HCC tissue was significantly higher than that in adjacent tissues (P<0.05). The expression of Beclin1 in HCC tissues and adjacent tissues was 0.83±0.15, 0.18±0.03, respectively, and the difference was statistically significant (P<0.05). Conclusion HBx and Beclin1 are highly expressed in HCC, and they interact and participate in the development of HCC. Combined detection of HBx and Beclin1 is of great value in judging the occurrence and metastasis of HCC. It is a candidate target gene for the treatment of HCC.