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研究了As2O3纳米粒的制备方法、表征及其抗肝癌细胞的作用.采用溶胶凝胶法制备As2O3纳米粒,并用透射电镜、能谱仪和图像分析仪等方法对其进行表征及特性检测.通过MTT法和流式细胞仪法研究了不同浓度As2O3纳米粒对人肝癌细胞株的影响,并与传统的As2O3溶液进行了比较.实验中制备了2种粒径大小的As2O3纳米粒子,其平均直径分别为80nm和40nm,通过能谱仪的测试证实所制备的纳米粒为As2O3,且无其他成分.体外细胞实验发现,As2O3纳米粒处理细胞48h后,人肝癌细胞的存活率明显低于同浓度的As2O3溶液处理组(P<0 05).研究结果显示,通过溶胶凝胶法可将As2O3制备成纳米粒子;体外细胞实验表明,与传统的As2O3溶液相比,As2O3 纳米粒子可对肿瘤细胞产生更强细胞毒作用.
As2O3 nanoparticles were prepared and characterized as well as their anti-hepatocarcinoma cell activity.As2O3 nanoparticles were prepared by sol-gel method and characterized by transmission electron microscopy, energy dispersive spectroscopy and image analyzer MTT and flow cytometry were used to study the effect of different concentrations of As2O3 nanoparticles on human hepatocarcinoma cell lines.Compared with the traditional As2O3 solution, two kinds of As2O3 nanoparticles were prepared and the average diameter Respectively, 80nm and 40nm, the results of energy dispersive spectroscopy test showed that the prepared nanoparticles were As2O3, and no other components.In vitro experiments showed that As2O3 nanoparticles treated cells after 48h, the survival rate of human hepatoma cells was significantly lower than the same concentration As2O3 solution treatment group (P <0 05) .The results show that As2O3 can be prepared into nanoparticles by the sol-gel method; in vitro cell experiments showed that compared with the traditional As2O3 solution, As2O3 nanoparticles can produce tumor cells More cytotoxic effects.