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以早实薄皮核桃‘绿岭’成熟种胚诱导出的无叶腋芽为外植体,MS为基本培养基,通过调节激素种类和浓度配比,初步研究了‘绿岭’核桃种胚无叶腋芽快繁体系。比较试验结果表明:最佳种胚萌发诱导培养基为MS+IBA 1.0mg/L+6-BA 1.0mg/L+蔗糖30g/L+琼脂6g/L;种胚无叶腋芽伸长培养基为MS+6-BA 2.0mg/L+IAA 1.0mg/L+GA31.5mg/L+蔗糖30g/L+琼脂6g/L。生根采用两步生根法:离叶柄基部2 mm切的茎段先在DKW+IBA 8.0mg/L+蔗糖30g/L+琼脂6.0g/L暗培养20d,然后转入DKW+蔗糖30g/L+琼脂6g/L培养基中进行光照培养,生根率为50%。诱导生根的组培苗移栽到大田后成活率为33.3%。
Leafless axillary buds induced by mature embryo of 'Green Ridge' of early real thin-walled walnut were used as explants. MS was used as basic medium. By adjusting hormone type and concentration ratio, Axillary buds rapid propagation system. The results showed that the optimal medium for induction of embryo germination was MS + IBA 1.0 mg / L + 6-BA 1.0 mg / L + sucrose 30 g / L + agar 6 g / L and embryoless leaf axillary bud elongation medium was MS + 6-BA 2.0 mg / L + IAA 1.0 mg / L + GA 31.5 mg / L + Sucrose 30 g / L + Agar 6 g / L. Roots were rooted in 2 steps and rooted at 2 mm from the base of petioles. The stems were first cultured for 20 days in DKW + IBA 8.0 mg / L + sucrose 30 g / L + agar 6.0 g / L and then transferred to DKW + sucrose 30 g / L + agar 6 g / L Culture medium for light, rooting rate of 50%. Induction of rooting of tissue culture seedlings transplanted to the field after the survival rate was 33.3%.