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目的:探讨苦参注射液对肺癌Lewis细胞的增殖、细胞周期、凋亡的影响。方法:采用MTT法及流式细胞技术,观察在不同浓度的苦参注射液处理24、48 h后,Lewis细胞增殖及细胞周期及凋亡的变化。结果:肺癌Lewis细胞在苦参注射液作用下表现出增殖抑制,且呈现剂量、时间依赖趋势;经处理后的肺癌Lewis细胞周期阻滞于G1期;在苦参注射液促进Lewis细胞凋亡。结论:苦参注射液能有效将肺癌Lewis细胞周期阻滞在G0/G1期,使其细胞增殖率显著降低,同时提高其细胞凋亡率。
Objective: To investigate the effects of matrine injection on the proliferation, cell cycle and apoptosis of Lewis lung cancer cells. Methods: MTT and flow cytometry were used to observe the changes of Lewis cell proliferation, cell cycle and apoptosis after treated with different concentrations of matrine for 24 and 48 hours. Results: Lewis lung cancer cells showed inhibition of proliferation under the action of Sophora flavescens injection in a dose-and time-dependent manner. Lewis lung cell cycle of the treated lung cancer cells was arrested in G1 phase. The Kushen injection promoted Lewis cell apoptosis. Conclusion: Kushen injection can effectively block the Lewis cell cycle of lung cancer in G0 / G1 phase, which can significantly reduce the cell proliferation rate and increase the apoptosis rate.