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利用两个对炭疽菌叶枯病高抗的苹果品种(系)‘富士’和‘QF-2’及两个高感品种‘金冠’和‘嘎拉’为亲本配制了4个杂交群体‘富士’ב金冠’,‘金冠’ב富士’,‘嘎拉’ב富士’,‘富士’בQF-2’。以F_1群体植株为试验材料,对苹果炭疽菌叶枯病的抗性进行鉴定评价和遗传分析。结果表明,4个群体中抗、感植株的分离比分别符合1:1,1:1,0:1和1:0的理论比值,初步推测苹果抗炭疽菌叶枯病性状受隐性单基因控制,抗病基因型为rr,感病基因型为RR和Rr。以‘金冠’ב富士’F_1群体为试材,采用分离群体分组分析(BSA)方法,通过对均匀覆盖苹果染色体组的500对SSR引物的筛选,获得了一个与抗病性状相关的分子标记S0506206-24,该标记与抗性基因位点的连锁距离为9.8 cM.
Four hybrids were used as parents for two apple cultivars (lines) ’Fuji’ and ’QF-2’ and two highly susceptible cultivars ’Golden Delicious’ and ’Gala’, which were resistant to anthrax leaf blight. Fuji ’×’ Golden Delicious’, ’Golden Delicious’ ×’ Fuji ’,’ Gala ’×’ Fuji ’,’ Fuji ’×’ QF-2 ’. F_1 population was used as experimental material to appraise and analyze the resistance of apple anthracis leaf blight. The results showed that the isolation ratios of resistant and susceptible plants in four populations were respectively in line with the theoretical ratios of 1: 1, 1: 1, 0: 1 and 1: 0. It was preliminarily presumed that the resistance to anthrax in apple was affected by recessive single gene Control, disease-resistant genotype rr, susceptible genotype RR and Rr. A total of 500 SSR primers were used to screen apple genomes in the ’Golden Delicious’ × ’Fuji’ F_1 population. The molecular markers related to disease resistance were obtained by using the segregation group analysis (BSA) method. S0506206-24. The linkage distance between the marker and the resistance gene locus was 9.8 cM.