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利用平板计数、微生物量碳和末端标记限制性片段长度多态性分析(terminal restriction fragment length polymorphism,T-RFLP)相结合的方法研究了2株生防菌哈茨木霉Th2和T4对甜瓜根围土壤微生态的影响。结果表明,生防菌Th2和T4对根围土壤细菌有明显的促生作用,而对真菌和放线菌有明显的抑制作用。T-RFLP数据统计学分析表明,Th2和T4的引入可以增加土壤细菌的多样性。其中对开花期土壤细菌菌群影响最显著,Th2对TRF251和TRF513菌群有显著的促生作用,含量是对照的3.7倍和4.5倍,T4对TRF251和TRF513菌群含量也比对照高4倍和5.6倍,而2株生防菌对TRF60菌群均有显著的抑制作用,其含量分别为对照的51.6%和64.3%。在果熟期生防菌对土壤细菌菌群的影响减弱,表明土壤的缓冲功能发挥了作用。研究结果还表明,Th2和T4对土壤细菌菌群有着相似的促生和抑制作用,且不会破坏土壤微生态的稳定性。
The effects of two pathogenic strains of T. harzianum Th2 and T4 on the root area of melon were studied using the combination of plate count, microbial biomass carbon and terminal restriction fragment length polymorphism (T-RFLP) Effects of soil microecology. The results showed that biocontrol agents, Th2 and T4, significantly promoted the growth of bacteria in rhizosphere soils, but significantly inhibited fungi and actinomycetes. Statistical analysis of T-RFLP data showed that the introduction of Th2 and T4 increased soil bacterial diversity. Among them, the most significant effect was on the soil bacterial flora at flowering stage. Th2 had a significant promoting effect on TRF251 and TRF513 colonies, which were 3.7 times and 4.5 times higher than that of control. T4 also had 4 times more TRF251 and TRF513 And 5.6 times, respectively, while two strains of biocontrol bacteria had a significant inhibitory effect on the TRF60 flora, which were respectively 51.6% and 64.3% of the control. The effect of biocontrol bacteria on soil bacterial flora during fruit maturity was weakened, suggesting that soil buffer function played a role. The results also show that Th2 and T4 have similar growth-promoting and inhibiting effects on soil bacterial flora, and do not destroy the stability of soil micro-ecology.