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目的:探讨β-catenin和DKK-3蛋白在宫颈鳞癌中的表达及相关性。方法:采用免疫组织化学方法 (SP法)检测20例正常宫颈组织、36例宫颈上皮内瘤变(CIN)及72例宫颈鳞癌组织中β-catenin蛋白的异常表达和DKK-3蛋白的阳性表达,并分析两种蛋白表达与宫颈鳞癌临床参数的关系。结果:DKK-3在正常宫颈、CIN及宫颈鳞癌组织中阳性表达率分别为100.00%、61.11%、36.11%,差异有统计学意义(P<0.001)。β-catenin在正常宫颈、CIN及宫颈癌组织中异常表达率分别为20.00%、52.78%、80.56%,差异有统计学意义(P<0.001)。DKK-3和β-catenin蛋白在宫颈鳞癌中的表达与临床分期、肿瘤分化程度及有无淋巴结转移有关(P<0.05)。宫颈鳞癌中DKK-3的阳性表达与β-catenin的异常表达呈负相关(r=-0.507,P=0.000)。结论:DKK-3表达减少或缺失与宫颈鳞癌的发病有相关性。
Objective: To investigate the expression and correlation of β-catenin and DKK-3 protein in cervical squamous cell carcinoma. Methods: The abnormal expression of β-catenin protein and DKK-3 protein in 20 cases of normal cervical tissue, 36 cases of cervical intraepithelial neoplasia (CIN) and 72 cases of cervical squamous cell carcinoma were detected by immunohistochemistry (SP method) Expression, and analyze the relationship between the expression of two proteins and clinical parameters of cervical squamous cell carcinoma. Results: The positive rates of DKK-3 in normal cervix, CIN and cervical squamous cell carcinoma tissues were 100.00%, 61.11% and 36.11%, respectively, with significant difference (P <0.001). The abnormal expression rates of β-catenin in normal cervix, CIN and cervical cancer tissues were 20.00%, 52.78% and 80.56%, respectively, with significant difference (P <0.001). The expression of DKK-3 and β-catenin in cervical squamous cell carcinoma was correlated with clinical stage, tumor differentiation and lymph node metastasis (P <0.05). The positive expression of DKK-3 in cervical squamous cell carcinoma was negatively correlated with the abnormal expression of β-catenin (r = -0.507, P = 0.000). Conclusion: The decrease or loss of DKK-3 expression is correlated with the incidence of cervical squamous cell carcinoma.