构建大豆fad3c基因的沉默载体,旨在为培育生物安全的高油酸低亚麻酸转基因大豆奠定基础。从高蛋白大豆黑农35中克隆大豆种子特异表达启动子GY1;从高油大豆黑农37中克隆fad2-1b基因内含子1;从5个转基因受体大豆中克隆fad3c基因片段,作为正向臂和反向臂。构建筛选标记基因为bar,启动子为GY1,内含子为fad2-1b基因内含子1的双T-DNA载体。将双T-DNA载体转化大肠杆菌DH5α,酶切和序列分析鉴定表明扩增得到的重组质粒正确,命名为pDT-GFAD3I。 Construction of Soybean fad3c Gene Silencing Vector to Establish a Foundation for the Development of Biosafety-High Oleic Low Linolenic Soybean. Cloning of soybean seed-specific promoter GY1 from high-protein soybean Heinong 35; Cloning of fad2-1b intron 1 from high-oil soybean Heinong 37; Cloning of fad3c gene fragment from 5 transgenic soybean lines as positive Arm and reverse arm. Construction of double T-DNA vector with bar marker, promoter GY1 and intron 1 intron 1 of fad2-1b gene. The double T-DNA vector was transformed into E. coli DH5α. The digestion and sequence analysis showed that the recombinant plasmid was correct and named as pDT-GFAD3I.