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目的:探讨落花生枝叶水煎液对大鼠脑缺血再灌注损伤(CI-RI)的潜在神经保护作用及其可能机制。方法:将大鼠随机分为对照组、假手术组、模型组以及落花生枝叶低[50 g/(kg·d)]、中[100 g/(kg·d)]、高剂量[150 g/(kg·d)]组,采用改良线栓法闭塞大脑中动脉(MCAO)制备大鼠局灶性CI-RI模型,通过神经缺损行为评分、脑梗死面积测定以及脑组织丙二醛(MDA)、活性氧类(ROS)、谷胱甘肽过氧化物酶(GPx)和超氧化物歧化酶(SOD)等生化指标检测来评价落花生枝叶水煎液的神经保护作用。结果:与模型组相比,落花生枝叶中、高剂量组能显著减轻神经缺损行为体征评分[分别为(2.27±0.80)、(1.93±0.80)和(1.73±0.80)分]、缩小脑梗死体积[分别为(42.46%±6.31%)、(32.20%±7.87%)和(24.80%±4.91%)],同时降低脑组织MDA[(519.66±54.31)、(445.87±12.05)和(303.98±36.03)nmol/mg蛋白]和ROS含量[(2.84±0.12)、(2.10±0.10)和(1.37±0.04)荧光强度/mg蛋白],提高GPx[(32.47±1.92)(66.50±6.04)和(86.39±6.35)U/mg蛋白]和SOD酶活力[(72.53±11.27)、(165.14±14.05)和(258.62±39.21)U/mg蛋白]。结论:落花生枝叶中、高剂量对大鼠CI-RI有潜在的神经保护作用,其作用机制与抗氧化应激有关。
Objective: To explore the potential neuroprotective effect of peanut branches and leaves decoction on cerebral ischemia-reperfusion injury (CI-RI) in rats and its possible mechanism. Methods: The rats were randomly divided into control group, sham operation group, model group and low pea [50 g / (kg · d)], medium [100 g / (kg · d) (kg · d)]. The model of focal CI-RI was established by occlusion of the middle cerebral artery (MCAO) by modified thread occlusion. The neurological deficit score, infarct size, , Reactive oxygen species (ROS), glutathione peroxidase (GPx) and superoxide dismutase (SOD) were measured to evaluate the neuroprotective effect of decoction of peanut branches and leaves. Results: Compared with the model group, the middle and high dose groups of peanut branches could significantly reduce the score of neurological deficits (2.27 ± 0.80, 1.93 ± 0.80 and 1.73 ± 0.80, respectively), and reduce the volume of cerebral infarction (42.46% ± 6.31%, (32.20% ± 7.87%) and (24.80% ± 4.91%, respectively]] and decreased MDA [(519.66 ± 54.31), (445.87 ± 12.05) and (303.98 ± 36.03 (32.47 ± 1.92) (66.50 ± 6.04) and (86.39) nmol / mg protein] and ROS content (2.84 ± 0.12, 2.10 ± 0.10 and 1.37 ± 0.04 fluorescence intensity / mg protein) ± 6.35) U / mg protein] and SOD activity [(72.53 ± 11.27), (165.14 ± 14.05) and (258.62 ± 39.21) U / mg protein]. CONCLUSION: Middle and high dose of peanut leaves have potential neuroprotective effect on CI-RI in rats, and its mechanism is related to anti-oxidative stress.