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目的通过炎症相关性结肠癌小鼠模型研究生育三烯酚联合奥沙利铂用药对结肠癌小鼠免疫功能的调节作用。方法选用5周龄CD1-ICR小鼠100只,按体质量随机分为空白对照组(Control)、肿瘤模型对照组(AOM/DSS)、奥沙利铂干预组(AOM/DSS+3 mg/kg体质量OXA)、100 mg/kg生育三烯酚干预组(AOM/DSS+100 mg/kg T3)和联合作用组(AOM/DSS+3 mg/kg OXA+100 mg/kg T3),每组20只,连续观察20周。实验期间,每周记录小鼠体质量;实验结束时,记录小鼠空腹体质量,称量脾脏及结肠,计算其脏器系数,观察小鼠脾脏及结肠组织病理学改变情况;检测各组小鼠外周血中TNF,IL-6,IL-4,IL-2的表达水平。结果与Control组相比,AOM/DSS组脾脏及结肠脏器系数显著增高,P<0.05,差异有统计学意义;与AOM/DSS组相比,AOM/DSS+OXA和AOM/DSS+OXA+T3组脾脏器系数显著下降,P<0.05,差异有统计学意义;与AOM/DSS组相比,AOM/DSS+OXA+T3组结肠脏器系数显著下降,P<0.05,差异有统计学意义。与Control组相比,AOM/DSS组外周血中血肿瘤坏死因子(tumor necrosis factor,TNF)、白介素(interleukin,IL)-6水平显著升高,P<0.05,差异有统计学意义;与AOM/DSS组相比,AOM/DSS+OXA、AOM/DSS+T3与AOM/DSS+OXA+T3组外周血TNF水平下降,AOM/DSS+OXA、AOM/DSS+OXA+T3组外周血IL-6水平下降,P<0.05,差异有统计学意义;与Control组相比,AOM/DSS组外周血IL-4水平显著降低,P<0.05,差异有统计学意义。结论生育三烯酚和奥沙利铂均可改善炎症相关性结肠癌小鼠免疫功能,抑制肿瘤发展。
OBJECTIVE: To study the regulatory effect of tocotrienol and oxaliplatin on the immune function of colon cancer in mice by inflammation-related mouse model of colon cancer. Methods 100 100-week-old CD1-ICR mice were randomly divided into control group, AOM / DSS group and AOM / DSS + 3 mg / kg body weight OXA), AOM / DSS + 100 mg / kg T3 and AOM / DSS + 3 mg / kg OXA + 100 mg / kg T3 at 100 mg / Group of 20, continuous observation of 20 weeks. During the experiment, the body weight of mice was recorded weekly. At the end of the experiment, the fasting body mass of mice was recorded, the spleen and colon were weighed, the organ coefficient was calculated, the histopathological changes of spleen and colon were observed, The levels of TNF, IL-6, IL-4 and IL-2 in peripheral blood of rats were detected. Results The AOM / DSS + OXA and AOM / DSS + OXA + AXA + DSS + AXA + Compared with AOM / DSS group, AOM / DSS + OXA + T3 group significantly decreased the index of colonic organ, P <0.05, the difference was statistically significant . Compared with Control group, the levels of tumor necrosis factor (TNF) and interleukin (IL) -6 in peripheral blood of AOM / DSS group were significantly increased (P <0.05) The levels of TNF in peripheral blood of AOM / DSS + OXA, AOM / DSS + T3 and AOM / DSS + OXA + T3 groups decreased compared with that of AOM / DSS + OXA + 6 level decreased, P <0.05, the difference was statistically significant; compared with the Control group, the level of IL-4 in peripheral blood of AOM / DSS group was significantly decreased, P <0.05, the difference was statistically significant. Conclusion Both tocotrienols and oxaliplatin can improve immune function and inhibit tumor development in mice with inflammation-associated colon cancer.