血吸虫感染后的免疫机制为疫苗的研究提供了理论依据,从基因文库中筛选编码诱导血吸虫保护性免疫力的抗原克隆是血吸虫分子疫苗研究非常关键步骤之一[1—3]。本文用日本血吸虫感染兔血清(IRS)探针来筛选日本血吸虫cDNA文库,并鉴定融合蛋白的性质,为血吸虫分子疫苗的研制打下基 The immune mechanism after schistosoma infection provides a theoretical basis for the vaccine research. Screening of antigenic clones encoding the protective immunity against schistosoma from the gene library is one of the key steps in molecular vaccine research of schistosoma [1-3]. In this paper, Schistosoma japonicum infected rabbit serum (IRS) probe to screen the cDNA library of Schistosoma japonicum, and to identify the nature of the fusion protein, laying the foundation for the development of schistosome molecular vaccine