本文以香港巨牡蛎(Crassostrea hongkongensis)和太平洋牡蛎(C.gigas)为研究对象,进行牡蛎异源三倍体研究试验。在水温25℃条件下,香港巨牡蛎剥离卵子于海水中浸泡促熟1.5h后,与太平洋牡蛎精子受精,获得最高卵裂率为(24.40±3.79)%。受精后20min,采用盐度为10的低渗海水处理杂交受精卵25min,可获得68.65%的异源三倍体幼虫,孵化率为12.71%。异源三倍体幼虫的存活率和生长率均未表现出优势。受精后第9天,异源三倍体幼虫的存活率仅为(0.116±0.023)%,平均壳长为(103.50±0.85)(m;对照组的存活率和壳长分别为(42.17±2.74)%和(123.25±8.60)μm。幼虫在附着变态前死亡。 In this paper, Crassostrea hongkongensis and C. gigas from Hong Kong were used to study the heterotetraploid study of oysters. Under the conditions of 25 ℃ water temperature, the giant oyster of Hong Kong was (24.40 ± 3.79)% with the sperm of Pacific oyster after it was immersed in seawater for 1.5h. After 20min fertilization, the hybrid fertilized eggs were treated with hypotonic salinity of 10 for 25min to obtain 68.65% allotriploid larvae, with a hatching rate of 12.71%. Allotriploid larvae did not show any survival advantage and growth rate. On the 9th day after fertilization, the survival rate of allotriploid larvae was (0.116 ± 0.023)% and the average shell length was (103.50 ± 0.85) m (m; the survival rate and shell length of the control group were (42.17 ± 2.74 )% And (123.25 ± 8.60) μm, respectively.The larvae died before attachment metamorphosis.