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目的观察奥曲肽联合氟脲嘧啶脱氧核苷或顺铂对肝癌细胞株SMMC-7721凋亡的影响,并探讨其治疗机制。方法体外培养SMMC-7721肝癌细胞株,根据干预药物分为6组,分别为阴性对照组(A组)、氟脲嘧啶脱氧核苷组(B组)、顺铂组(C组)、奥曲肽组(D组)、奥曲肽+氟脲嘧啶脱氧核苷组(E组)与奥曲肽+顺铂组(F组),分别干预SMMC-7721细胞24,48,72,96h后,应用酶联免疫检测仪测定各组吸光值并统计细胞抑制率,采用免疫组织化学方法检测各组SMMC-7721细胞表面Fas及FasL蛋白表达变化。结果药物作用24,48,72,96h时,B,C,D,E,F组SMMC-7721肝癌细胞抑制率及Fas和FasL蛋白表达明显高于A组(P<0.05);E组较B组,F组较C组抑制率明显增加,Fas及FasL蛋白表达明显增强(P<0.05)。结论上调肿瘤细胞表面Fas及FasL的表达是奥曲肽协同氟尿嘧啶脱氧核苷或顺铂抑制SMMC-7721细胞增殖,发挥抗肿瘤作用的可能机制之一。
Objective To observe the effect of octreotide combined with fluoxetine or cisplatin on the apoptosis of hepatocellular carcinoma cell line SMMC-7721 and to explore its therapeutic mechanism. Methods SMMC-7721 hepatocellular carcinoma cell line was cultured in vitro and divided into 6 groups according to the interventional drugs: negative control group (group A), fluorouracil deoxynucleoside group (group B), cisplatin group (group C), octreotide group (D group), octreotide + fluorouracil deoxynucleoside group (E group) and octreotide + cisplatin group (F group). The SMMC-7721 cells were respectively treated for 24, 48, 72 and 96 hours, then detected by enzyme-linked immunosorbent assay The absorbance of each group was measured and the cell inhibitory rate was calculated. The expression of Fas and FasL protein in SMMC-7721 cells was detected by immunohistochemistry. Results The inhibitory rates of Fas and FasL protein expression in SMMC-7721 hepatocellular carcinoma cells in B, C, D, E and F groups were significantly higher than those in A group (P <0.05) at 24, 48, 72, Compared with group C, the inhibition rate of group F and F increased significantly, and the expression of Fas and FasL increased significantly (P <0.05). Conclusions Upregulation of Fas and FasL expression on tumor cells is one of the possible mechanisms by which octreotide synergizes with fluorouracil or cisplatin in inhibiting SMMC-7721 cell proliferation and exerting antitumor effects.