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Aim:To explore the cell specific existence of α9 AChR in the vestibular type Ⅱ haircells(VHC Ⅱ)of rats.Methods:To detect the expression of α9 AChR messengerRNA(mRNA)in the vestibular endorgans and single VHC Ⅱ of rats by using thereverse transcription polymerase chain reaction(RT-PCR)technique and the singlecell RT-PCR technique,respectively.Results:It was shown that α9 AChR mRNAwas detected in the vestibular endorgans.By using single-cell RT-PCR,mRNAencoding α9 AChR was also detected in the VHC Ⅱ of the rats.Sequence analysisof the PCR products confirmed identity to corresponding cDNA sequence in thepredicted region.Conclusion:We established a method which could effectivelydetect the cell specific expression of mRNA in an individual VHC.Present dataconfirm that α9 AChR mRNA is expressed in the VHC Ⅱ of rats and indicates thatα9 AChR may function as a mediator of efferent cholinergic signaling in mamma-lian VHC.
Aim: To explore the cell specific existence of α9 AChR in the vestibular type Ⅱ hair cells (VHC Ⅱ) of rats. Methods: To detect the expression of α9 AChR messengerRNA (mRNA) in the vestibular endorgans and single VHC Ⅱ of rats by using thereverse Results: It was shown that α9 AChR mRNA was detected in the vestibular endorgans. By using single-cell RT-PCR, mRNA encoding α9 AChR was also detected in the VHC II of the rats. Sequencing analysis of the PCR products confirmed identity to corresponding cDNA sequence in the predicted region. Confluence: We established a method which could effectively detect the cell specific expression of mRNA in an individual VHC. Present dataconfirm that α9 AChR mRNA is expressed in the VHC II of rats and that that α9 AChR may function as a mediator of efferent cholinergic signaling in mamma-lian VHC.