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目的观察非毒性剂量冬凌草甲素是否增加鼻咽癌细胞株HONE1对米托蒽醌的敏感性。方法采用MTT法使用不同浓度的冬凌草甲素处理HONE1细胞株,确定冬凌草甲素的非毒剂量区间;然后用非毒性剂量冬凌草处理细胞后用流式细胞术进行侧群表型检测,探索能显著降低侧群表型的冬凌草甲素浓度;参考冬凌草甲素非毒性剂量和能降低侧群表型的剂量得到冬凌草甲素用于联用米托蒽醌的浓度。结果 5μmol/L冬凌草甲素并未对HONE1细胞产生明显毒性,该浓度处理细胞24 h后,细胞侧群比例由3.32%降低至0.55%;米托蒽醌联用5μmol/L冬凌草甲素组HONE1细胞存活率较单用米托蒽醌组低(P<0.05);流式细胞术分析显示膜外排蛋白ABCG2含量降低。结论冬凌草甲素可以通过影响细胞ABCG2外排功能增加鼻咽癌细胞株HONE1对米托蒽醌的敏感性。
Objective To investigate whether non-toxic dose of oridonin can increase the sensitivity of nasopharyngeal carcinoma cell line HONE1 to mitoxantrone. Methods MTT method was used to treat HONE1 cells with different concentrations of oridonin to determine the non-toxic dose interval of oridonin. Then the cells were treated with non-toxic dose of rubescens and treated with flow cytometry Type detection and exploration can significantly reduce the side group phenotype of oridonin concentration; reference to oridonin nontoxic dose and dose can reduce the side population phenotype Oridonin for combination with mitoxantrone Quinone concentration. Results Oridonin 5 μmol / L did not produce any significant cytotoxicity on HONE1 cells. After 24 h treatment, the percentage of cells in the cells decreased from 3.32% to 0.55%. Mitoxantrone combined with 5 μmol / The survival rate of HONE1 in A group was lower than that in mitoxantrone alone group (P <0.05). Flow cytometry analysis showed that the content of ABCG2 was decreased. Conclusion Oridonin can enhance the sensitivity of nasopharyngeal carcinoma cell line HONE1 to mitoxantrone by affecting efflux of ABCG2 cells.