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目的建立稳定转染ndrg2基因的脑胶质瘤细胞系,以探索及研究该基因的功能。方法以低表达ndrg2基因的脑胶质瘤细胞系U251为材料,以ndrg2基因转染U251细胞系,经G418进行筛选后,挑取单克隆进行培养,用荧光显微镜观察、逆转录酶聚合酶链反应(RT-PCR)验证获得的表达细胞株。结果经转染和G418筛选后,荧光显微镜下见有明显表达ndrg2基因的细胞约占70%～80%,RT-PCR检测到转染基因ndrg2后细胞ndrg2的高表达,而转染空载体的细胞ndrg2低表达:结论成功建立了稳定转染基因ndrg2的脑胶质瘤细胞系U251,为进一步探讨该基因的功能奠定了一定基础。
Objective To establish a glioma cell line stably transfected with ndrg2 gene to explore and study the function of this gene. Methods The glioma cell line U251 with low expression of ndrg2 gene was used as the material. The U251 cell line was transfected with ndrg2 gene. After being screened by G418, the monoclonal antibody was used to culture the cells. Fluorescence microscopy, reverse transcriptase polymerase chain The expressed cell lines obtained were verified by RT-PCR. Results After transfection and screening with G418, about 70% ~ 80% of the cells expressed ndrg2 gene under fluorescence microscope. The high expression of ndrg2 gene was detected by RT-PCR and the expression of ndrg2 gene was higher in transfected empty vector Low expression of ndrg2 in cells: Conclusion The glioma cell line U251 stably transfected with ndrg2 gene was successfully established, which laid a foundation for further exploring the function of this gene.