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目的:观察超声波用于螺旋藻多糖提取的可行性,并对实验条件进行优化。方法:通过正交实验优化了用超声波法提取螺旋藻多糖的实验条件,并与单纯水浴提取法进行了比较。结果:螺旋藻干粉在pH11和60℃的水浴条件下超声波处理60min得到的螺旋藻多糖的量最多。与单纯水浴提取法相比,超声波法粗提物的提取率高(分别为3.175±0.214%和2.023±0.307%,P<0.05),体外抗肿瘤实验结果表明,两种提取方法得到的螺旋藻多糖对卵巢癌SKV3细胞的生长均有明显的抑制作用(MTT法抑瘤率分别为43.013±3.497%和39.265±3.293%),两种提取方法得到的螺旋藻多糖对SKV3细胞的生长的抑制效果大致相同(P>0.05)。结论:螺旋藻多糖提取的最佳方案是在pH 11和60℃的水浴条件下超声波处理60min,再静置60℃水浴60min的分离提取方案。
OBJECTIVE: To observe the feasibility of ultrasonic extraction of Spirulina polysaccharides and optimize the experimental conditions. Methods: The orthogonal experiment was used to optimize the experimental conditions for extracting Spirulina polysaccharides by ultrasonic method, and compared with the simple water bath extraction method. RESULTS: The amount of Spirulina polysaccharides was obtained by the ultrasonic treatment of Spirulina powder for 60 min at pH 11 and 60 °C. Compared with the simple water bath extraction method, the extraction rate of crude extracts by ultrasonic method was high (3.175±0.214% and 2.023±0.307%, respectively, P<0.05). The results of in vitro anti-tumor experiments showed that the polysaccharides obtained from the two extraction methods were polysaccharides of Spirulina platensis. The growth of ovarian cancer SKV3 cells was significantly inhibited (MTST inhibition rates were 43.013±3.497% and 39.265±3.293%, respectively). Spirulina polysaccharides obtained by the two extraction methods had a broad inhibitory effect on the growth of SKV3 cells. The same (P>0.05). Conclusion: The best solution for the extraction of Spirulina polysaccharides is ultrasonic treatment for 60 min at pH 11 and 60 °C in water bath, and 60 °C water bath for 60 min.