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为了改善避蚊胺(diethyltoluamide,以下简称DEET)在水中的溶解度,首先采用饱和溶液法制备了避蚊胺/β-环糊精(diethyltoluamide/β-cyclodextrin,以下简称DEET/β-CD)包合物,其结构通过差示扫描量热仪(DSC)和X-射线衍射仪(XRD)表征;再采用离子交联法,以氯化钙为交联剂,制备了避蚊胺/β-环糊精/O-羧甲基壳聚糖(diethyltoluamide/β-cyclodextrin/Ocarboxymethyl chitosan,以下简称DEET/β-CD/O-CMC)亲水性纳米胶囊,用紫外-可见分光光度计(UV)、激光粒度分析仪和扫描电子显微镜(SEM)考察其理化性质;最后,考察包合物和纳米胶囊的缓释性能。结果表明:DEET与β-CD以物质的量之比1:1进行了包合,β-CD的加入使DEET的溶解度增加约27倍,该纳米胶囊的包封率为(79.93±2.08)%,载药量为(12.11±0.09)%,纳米胶囊呈椭圆形,平均粒径为(230.50±11.34)nm,Zeta电位为(–19.08±0.99)m V,PDI为0.055。在Tris-HCl(p H=7.4)缓冲液中释放试验显示,DEET/β-CD/O-CMC亲水性纳米胶囊比DEET/β-CD包合物有更好的缓释性能,24 h累积释放率分别为27.04%和38.10%。
In order to improve the solubility of DEET (diethyltoluamide) in water, a DEET / β-cyclodextrin (DEET / β-CD) The structure was characterized by differential scanning calorimetry (DSC) and X-ray diffractometry (XRD). Using ion-crosslinking method and calcium chloride as the crosslinking agent, DEET / β-ring Dextrin / O-carboxymethyl chitosan (DEET / β-CD / O-CMC) hydrophilic nanocapsules were characterized by UV- Laser particle size analyzer and scanning electron microscopy (SEM) to examine its physical and chemical properties; Finally, the inclusion compound and nanocapsules sustained release performance. The results showed that the entrapment efficiency of DEET with β-CD was 1: 1 and the solubility of DEET was increased by 27-fold with the addition of β-CD. The encapsulation efficiency of the nanocapsules was (79.93 ± 2.08)%, (12.11 ± 0.09)%. The nanocapsules were oval. The mean diameter was (230.50 ± 11.34) nm. The Zeta potential was (-19.08 ± 0.99) mV. The PDI was 0.055. The release assay in Tris-HCl buffer (p H = 7.4) showed that the DEET / β-CD / O-CMC hydrophilic nanocapsules had better sustained release properties than DEET / β-CD inclusion complexes. The cumulative release rates were 27.04% and 38.10% respectively.