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目的:研究miR-215在结肠直肠癌(colorectal cancer,CRC)组织中的表达及其临床意义。方法:研究对象是我院2007年1月至6月的进展期CRC病人,从入选病人的石蜡包埋组织中提取microRNA,进行实时定量PCR检测。结果:共有42例CRC病人入选,男25例,女17例;直肠癌18例,结肠癌24例。miR-215在CRC组织中的表达水平低于配对的癌旁正常组织(P<0.001);CRC组织中miR-215下降不明显者的生存时间较长(P=0.035)、P53表达水平较高(r=0.556,P<0.001)。结论:miR-215可能成为CRC的预后标志物。
Objective: To investigate the expression of miR-215 in colorectal cancer (CRC) and its clinical significance. Methods: The subjects were advanced CRC patients from January 2007 to June 2007 in our hospital. MicroRNAs were extracted from paraffin-embedded tissues of selected patients for real-time quantitative PCR. Results: A total of 42 CRC patients were enrolled, including 25 males and 17 females; 18 rectal cancer patients and 24 colon cancer patients. The expression level of miR-215 in CRC tissues was lower than that in paired adjacent normal tissues (P <0.001). The survival time of miR-215significant in CRC tissues was longer (P = 0.035), P53 expression was higher (r = 0.556, P <0.001). Conclusion: miR-215 may be a prognostic marker of CRC.