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本文在多种基因组提取方法的基础上，针对红麻基因组提取中的难点即富含酚类、萜类、单宁、果胶等物质。提出了利用１％抗坏血酸保护单宁、多酚类物质不被褐化，避免了其褐化产物与ＤＮＡ形成不可逆的复合物，提高了ＤＮＡ得率；并且，提取缓冲液采用高ｐＨ条件下Ｎａ２ＣＯ３存在可加速果胶碱解，分离果胶等杂质，获得了高纯度基因组ＤＮＡ，适合ＰＣＲ扩增等分子生物学要求
In this paper, based on a variety of genomic extraction methods, the kenaf genomic extraction of the difficulties that is rich in phenols, terpenes, tannins, pectin and other substances. Proposed the use of 1% ascorbic acid to protect tannin, polyphenols are not browning, to avoid its browning products and DNA irreversible complex formation, improve the yield of DNA; and, the extraction buffer using high pH Na2CO3 Presence of accelerating pectin alkaline hydrolysis, separation of pectin and other impurities, obtained high purity genomic DNA, suitable for PCR amplification and other molecular biology requirements