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作者将流感病毒血凝素(HA)亚单位疫苗〔由甲型流感病毒NIB26(H3N2)制备〕与脂质体(蛋黄卵磷脂:胆固醇:三十二烷磷酸盐=4:5:1)混合,经下呼吸道免疫雌性小鼠,能诱导泌尿生殖道产生分泌型IgA抗体。 实验分4组进行。每组5只8～10周龄的雌性Balb/c小鼠。第1组于麻醉状态下鼻腔滴注50μl脂质体疫苗(全呼吸道免疫);第2组于未麻醉状态下鼻腔滴入20μl脂质体疫苗(上呼吸道免疫);第3组于麻醉状态下作气管切口,直接将50μl脂质体疫苗注入肺中;第4组肌肉注射或口服50μl疫苗作为对照组。肌肉或鼻腔接种的HA抗原均为5μg,脂质体剂量为1mg。免疫后4周,收集支气管洗液。连续三天以PBS冲洗阴道,收集洗液,合并每只小鼠的冲洗物。最后一次冲洗后,处死小鼠采血。用ELISA检测血清中抗HA特异性
The authors mixed the influenza virus hemagglutinin (HA) subunit vaccine [prepared from influenza A virus NIB26 (H3N2)] with liposomes (egg yolk lecithin: cholesterol: tridecyl phosphate = 4: 5: 1) , Via the lower respiratory tract immunization of female mice, can induce genitourinary secretion of IgA antibodies. The experiment was divided into 4 groups. Five female Balb / c mice, 8-10 weeks of age, were included in each group. Group 1 received intranasal instillation of 50 μl liposomal vaccine under nasal anesthesia (whole-airway immunization); Group 2, 20 μl of liposomal vaccine (upper respiratory tract) were intranasally instilled without anesthesia; group 3 under anesthesia For tracheostomy, 50 μl liposomal vaccine was injected directly into the lungs; Group 4 was injected intramuscularly or orally with 50 μl of vaccine as a control. The HA or nasal inoculated HA antigen was 5 μg and the liposome dose was 1 mg. Four weeks after immunization, bronchial washings were collected. The vagina was rinsed with PBS for three consecutive days, the washings were collected, and the washings of each mouse were combined. After the last rinse, mice were sacrificed and blood was collected. Anti-HA specificity in serum was detected by ELISA