论文部分内容阅读
目的 :建立夏天无中原阿片碱和延胡索乙素的含量测定方法 ,为夏天无质量标准的研究提供科学依据。方法 :采用高效液相色谱法分离并测定了中药夏天无中原阿片碱和延胡索乙素的含量。色谱条件 :SupelcosilLC 18 DB柱 ,以甲醇 乙酸钠 /冰乙酸缓冲溶液 (75∶2 5 ,pH5 .0 )为流动相 ;检测波长 2 85nm。 4 86型紫外检测器检测。 结果 :此方法线性良好 ,平均加样回收率分别为 98.0 4 % ,99.6 3%。结论 :本方法精密度高 ,分离度良好 ,具有实用性
Objective : To establish a method for determining the content of opiate and tetrahydropalmatine in summer without any reference to provide scientific basis for the study of summer without quality standards. Methods : The contents of opiate and tetrahydropalmatine in Chinese herbal medicine were determined by high performance liquid chromatography. Chromatographic conditions: SupelcosilLC 18 DB column, methanol-sodium acetate / glacial acetic acid buffer solution (75:25, pH5.0) as the mobile phase; detection wavelength of 285nm. Type 4 86 UV detector detection. Results : This method has a good linearity and the average recovery rate is 98.0 4 % and 99.6 3% respectively. Conclusion : This method has high precision, good resolution and practicality.