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目的探讨赫赛汀(Herceptin,HER)对HER2高表达胃癌细胞裸鼠移植瘤的抑制效应及其相应机制。方法将处于对数生长期的HER2细胞皮下注射到Balb/c裸鼠体内4w后,用HER处理裸鼠移植瘤模型3w,测量各组移植瘤的体积大小,同时观察移植瘤的组织结构。并利用免疫组化法检测移植瘤细胞Ki-67蛋白的表达变化,以检测移植瘤细胞的增殖活性。果HER作用组能显著减小HER2高表达胃癌细胞裸鼠移植瘤的体积并有效改变其组织病理结构,同时还能在体内显著抑制HER2阳性胃癌细胞的增殖活性。结论应用HER能够有效抑制HER2高表达胃癌细胞的作用。
Objective To investigate the inhibitory effect of Herceptin (HER) on HER2-overexpressing gastric cancer xenografts in nude mice and its corresponding mechanism. Methods HER2 cells in logarithmic growth phase were injected subcutaneously into Balb / c nude mice for 4 weeks. The nude mouse xenograft model was treated with HER for 3 weeks. The volume of the transplanted tumor in each group was measured, and the histological structure of the transplanted tumor was observed. The expression of Ki-67 protein in the tumor cells was detected by immunohistochemistry to detect the proliferation activity of the transplanted tumor cells. If the HER group can significantly reduce the volume of HER2-overexpressing gastric cancer xenografts in nude mice and effectively change its histopathology, it can also significantly inhibit the proliferation of HER2-positive gastric cancer cells in vivo. Conclusion The application of HER can effectively inhibit HER2 overexpression of gastric cancer cells.