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目的建立豚鼠感染EB病毒(Epstein-Barr virus,EBV)动物模型,分析EBV对豚鼠的易感性。方法将EBV经鼻腔和口腔感染豚鼠,建立感染EBV动物模型。提取分离的豚鼠淋巴细胞总RNA,巢式PCR法检测EBNA1及BcLF1基因mRNA的表达水平;ELISA法检测豚鼠血清中抗EBV IgG抗体水平的变化。取豚鼠肝脏、肺、脾脏、腮腺进行病理分析。结果10只模型组豚鼠中有6只检测到BcLF1基因mRNA的表达,其中有4只同时检测到了EBNA1和BcLF1基因mRNA的表达;经双酶切及测序鉴定,扩增的EBNA1及BcLF1基因大小与预期相符。10只豚鼠血清中VCA IgG和EBNA-IgG抗体水平均有所升高。模型组中有5只豚鼠的肝脏、肺、脾脏、腮腺的组织发生不同程度的病变。结论豚鼠可作为EBV感染动物模型的备选动物。
Objective To establish an animal model of Epstein-Barr virus (EBV) infection in guinea pigs and analyze the susceptibility of EBV to guinea pigs. Methods EBV was intranasally and orally infected with guinea pigs to establish an animal model of EBV infection. The total RNA of isolated guinea pig lymphocytes was extracted. The expression of EBNA1 and BcLF1 mRNA was detected by nested PCR. The level of anti-EBV IgG antibody in guinea pig serum was detected by ELISA. Take guinea pig liver, lung, spleen, parotid gland for pathological analysis. Results The mRNA expression of BcLF1 was detected in 6 out of 10 guinea pigs, including 4 EBNA1 and BcLF1 mRNAs. The double-digested and sequenced DNA fragments of EBNA1 and BcLF1 Expected match. VCA IgG and EBNA-IgG antibody levels increased in 10 guinea pigs. In the model group, there were 5 guinea pigs with varying degrees of lesions in the liver, lung, spleen and parotid gland tissues. Conclusion Guinea pigs can be used as an animal model of EBV infection.