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延胡索酸毒性小,可作为食品添加剂。目前日本厚生省的食品中延胡索酸的公定测定方法是以三甲基硅烷衍生物的形式进行色谱测定,或者用苹果酸脱氢酶的酶化学法测定,其操作烦琐。作者研究了反相HPLC测定延胡索酸的方法,并对市售的18种苹果酸及苹果饮料中的微量延胡索酸进行了测定。试剂延胡索酸标准物质:特级品,延胡索酸的纯度需经HPLC测定,即确认色谱溶出峰为单一峰。称取一定重量的延胡索酸用水溶解配成1.0×10~(-2)M的标准溶液,按需要稀释成一定的浓度。
Fumarate small toxicity, can be used as food additives. At present, Japan Ministry of Health and Welfare fumarate determination of the food in the form of trimethylsilyl derivatives of the chromatographic determination, or by malate dehydrogenase enzyme chemistry, the cumbersome operation. The authors studied the method for the determination of fumaric acid by reversed phase HPLC and the determination of trace fumaric acid in 18 commercially available malic acid and apple drinks. Reagent fumarate standard substance: special grade, fumaric acid purity to be determined by HPLC to confirm that the chromatographic dissolution peak is a single peak. Weigh a certain weight of fumaric acid dissolved in water dubbed 1.0 × 10 ~ (2) M standard solution, diluted to a certain concentration as needed.