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目的探讨运动训练、表没食子儿茶素没食子酸酯(EGCG)单独干预及联合应用对2型糖尿病大鼠肾脏血管通透性和肾TLR4、NF-κBp65、IL-1β蛋白表达量的影响。方法通过高脂喂养加腹腔注射小剂量链脲佐菌素(STZ)构建2型糖尿病SD大鼠模型。将造模成功的56只大鼠随机分为糖尿病对照组(D)、糖尿病给药组(DY)、糖尿病运动组(DE)、糖尿病运动给药组(DEY),每组14只,另设正常对照组(C)12只。运动组大鼠进行12周不负重游泳训练,每周训练5 d。EGCG给药组大鼠每周灌胃7 d。实验结束后检测大鼠FBG、尿蛋白、UAER、TLR4、NF-κBp65、IL-1β蛋白和肾脏血管通透性变化,并对实验结果进行统计分析。结果与正常对照组(C)相比,糖尿病对照组(D)的FBG、尿蛋白、UAER、TLR4、NF-κBp65、IL-1β蛋白和肾脏血管通透性均显著升高(P<0.01);糖尿病给药组(DY)、糖尿病运动组(DE)、糖尿病运动给药组(DEY)的FBG、尿蛋白、UAER、TLR4、NF-κBp65、IL-1β蛋白和肾脏血管通透性较糖尿病对照组(D)均显著降低(P<0.05或P<0.01);糖尿病运动给药组(DEY)FBG、UAER、TLR4、NF-κBp65、IL-1β蛋白表达量和肾血管通透性均显著低于糖尿病给药组(DY)(P<0.05或P<0.01),糖尿病运动给药组(DEY)肾脏指数、尿蛋白、UAER、TLR4、NF-κBp65、IL-1β蛋白表达量和肾血管通透性均显著低于糖尿病运动组(DE)(P<0.05)。结论 2型糖尿病大鼠存在肾组织炎性因子表达增多,运动训练和EGCG可改善2型糖尿病大鼠肾脏炎性损伤,抑制TLR4信号通路。
Objective To investigate the effects of exercise training, epigallocatechin gallate (EGCG) alone and in combination on renal vascular permeability and protein expression of TLR4, NF-κBp65 and IL-1β in type 2 diabetic rats. Methods SD rats with type 2 diabetes were induced by high fat diet and intraperitoneal injection of low dose streptozotocin (STZ). Fifty-six rats were randomly divided into diabetic control group (D), diabetic group (DY), diabetic exercise group (DE) and diabetic exercise group (DEY) Normal control group (C) 12. Rats in exercise group received 12-week non-weight-bearing swimming training and trained for 5 days per week. Rats in EGCG group were given gavage for 7 days every week. After the experiment, the changes of vascular permeability of FBG, urinary protein, UAER, TLR4, NF-κBp65, IL-1β protein and kidney in rats were detected, and the experimental results were statistically analyzed. Results Compared with control group (C), FBG, urinary protein, UAER, TLR4, NF-κBp65, IL-1β protein and renal vascular permeability in diabetic control group were significantly increased (P <0.01) ; Diabetic group (DY), Diabetic exercise group (DE), Diabetic exercise group (DEY), FBG, urinary protein, UAER, TLR4, NF-κBp65, IL- (P <0.05 or P <0.01). The protein expression of FBG, UAER, TLR4, NF-κBp65 and IL-1β in diabetic exercise group (DEY) and renal vascular permeability were all significantly higher than those in control group (P <0.05 or P <0.01). The expression of renal index, urinary protein, UAER, TLR4, NF-κBp65 and IL-1β in diabetes mellitus patients with exercise-induced diabetic nephropathy Permeability was significantly lower than that of diabetic exercise group (DE) (P <0.05). Conclusion The expression of inflammatory cytokines in the kidney of type 2 diabetic rats increased. Exercise training and EGCG could improve the renal inflammatory injury and inhibit the TLR4 signaling pathway in type 2 diabetic rats.