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本文报告一种胶体薄膜过滤法,只需靶组织50毫克样品即可测知其中所含有女性素受体的量,所得结果完全可与蔗糖梯度离心法相比。本文报告了宫内膜细胞细胞匀浆对[~3H]17B-雌二醇特殊亲和性的测定结果。其它非特异性成分未做。作者认为本法的全部步骤在临床上完全适用于测定自乳腺和宫内膜肿瘤采取的活检组织,从而能够预知该肿瘤对内分泌的依赖
This article reports a colloidal membrane filtration method that can detect the amount of feminine receptors contained in a target tissue of 50 mg. The results obtained can be compared with sucrose gradient centrifugation. This article reports the results of measuring the specific affinity of [~3H]17B-estradiol in cell homogenates of endometrial cells. Other non-specific components were not done. The author believes that all the steps of this method are clinically applicable to the determination of biopsy tissue taken from breast and endometrium tumors, so as to predict the tumor’s dependence on endocrine.