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目的通过改进供试品溶液的制备过程,采用紫外检测器建立测定高丽红参含量的高效液相色谱(HPLC)法,以改进进口药材标准中的含量测定方法。方法色谱条件为Agilent poroshell C_(18)柱(4.6 mm×75 mm,5μm);以乙腈-水为流动相进行梯度洗脱,流速为0.7 mL·min~(-1),检测波长为203 nm,柱温为25℃。供试品溶液采用水饱和正丁醇加热回流提取进行制备。结果改进方法的人参皂苷Rg_1在0.920 9~9.208 5μg、人参皂苷Re在0.928 0~9.279 8μg、人参皂苷Rb_1在0.955 2~9.551 8μg范围内与峰面积呈良好的线性关系;人参皂苷Rg_1、人参皂苷Re、人参皂苷Rb_1平均加样回收率分别为100.9%,102.5%,101.7%(n=6)。本方法结果比原标准方法的结果略高,相对偏差均小于5%。结论改进后的方法操作简单准确,重现性好,能提高检测效率。
OBJECTIVE To establish a HPLC method for the determination of ginseng in ginseng by improving the preparation process of the sample solution and using UV detector to improve the method of determination of the contents of imported herbs. Methods The chromatographic conditions were Agilent poroshell C_ (18) column (4.6 mm × 75 mm, 5 μm). The mobile phase consisted of acetonitrile and water. The flow rate was 0.7 mL · min -1 and the detection wavelength was 203 nm The column temperature was 25 ℃. The test solution was saturated water butanol reflux extraction was prepared. Results The results showed that there was a good linear relationship between ginsenoside Rg_1 and ginsenoside Rg_1 in the range of 0.920 9 ~ 9.208 5μg, ginsenoside Re 0.928 0 ~ 9.279 8μg, ginsenoside Rb_1 in the range of 0.955 2 ~ 9.551 8μg, Re, ginsenoside Rb_1 average recovery was 100.9%, 102.5%, 101.7% (n = 6). The result of this method is slightly higher than that of the original standard method, and the relative deviation is less than 5%. Conclusion The improved method is simple, accurate, reproducible and can improve the detection efficiency.