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1988年室内试验结果证明:芜菁夜蛾线虫的5个品系Agriotos、All、Mexican、NC 116、Florida对蛀干害虫小木蠹蛾幼虫有很高的侵染力;但不侵染蛹。用Agriotos品系作试验,剂量和温度影响小木蠹蛾的死亡率和致死速度。当剂量大于25条线虫/木蠹蛾幼虫时,死亡率可达92%以上,同一剂量对一年、二年生幼虫的死亡率差异不显著。在15-30℃的范围内,线虫均能侵染和杀死寄主,但30℃不能在寄主尸体内繁殖。25℃为线虫浸染的最适宜温度,寄主死亡率最高,死亡速度也最快。一头5龄和7龄木蠹蛾幼虫能繁殖侵染期线虫16万和48万头。在10℃低温中,线虫不能侵染寄主,但并不死亡,当移入适宜的温度后,能迅速恢复活动。在35℃高温中,线虫则很快死亡。
In 1988, laboratory tests showed that Agriotos, All, Mexican, NC116 and Florida of V. elegans had high infectivity on the larvae of the moth borer moth, but did not infiltrate the pupae. The Agriotos strain was used to test the effects of dose and temperature on the mortality and lethality of C. minor. When the dose is more than 25 nematodes / C. viscosus larvae, the mortality rate can reach more than 92%. The same dose has no significant difference in the mortality rate of one year and two year larvae. Nematodes can infect and kill the host within the range of 15-30 ° C, but not at 30 ° C. 25 ℃ is the most suitable temperature for nematode dissemination, with the highest death rate and the fastest death rate. A 5th instar and 7th instar larvae of P. cicadae can propagate 160 000 and 480 000 nematodes during infection. Nematodes can not invade the host at low temperatures of 10 ° C, but do not die, and resume activity quickly when moved to the appropriate temperature. At 35 ° C, nematodes die quickly.