论文部分内容阅读
肌钙蛋白（Ｔｎ）Ｉ有着极强的心肌特异性，心肌肌钙蛋白是唯一存在于心肌细胞中的异构体，可以通过检测患者血液中的心肌ＴｎＩ含量来确诊急性心肌梗塞。根据在钙离子存在下，ＴｎＣ和ＴｎＩ特异结合的特性，可以利用亲和层析提取纯化ＴｎＩ。根据ＴｎＩ从亲和层析柱的穿透曲线，对Ａｒｎｏｌｄ等人提出的孔内扩散模型进行了分析，证明孔内扩散过程不是此亲和层析过程中的限速步骤。在缓冲液体系（ｐＨ８．０）中，提出了包括以一个正向二级、逆向一级来描述ＴｎＣ—ＴｎＩ反应速率方程，并用实验验证了该模型的正确性，从而首次阐明了ＴｎＣ—ＴｎＩ提取纯化体系的动力学机制，揭示了该过程的限速步骤为ＴｎＣ—ＴｎＩ反应过程。
Troponin (Tn) I has a strong cardiac specificity. Cardiac troponin is the only isoform present in cardiomyocytes. Acute myocardial infarction can be diagnosed by measuring myocardial TnI levels in the patient’s blood. Purified TnI can be extracted by affinity chromatography based on the specific binding of TnC and TnI in the presence of calcium ions. According to the breakthrough curve of TnI from the affinity chromatography column, the intrapore diffusion model proposed by Arnold et al. Is analyzed, and it is proved that the intrapore diffusion process is not a rate-limiting step in this affinity chromatography process. In the buffer system (pH8.0), the reaction rate equation of TnC-TnI was described in a positive second-order and reverse order. The correctness of the model was verified by experiment, and the TnC-TnI The kinetic mechanism of the purification system was extracted and the rate-limiting step of the process was revealed as TnC-TnI reaction process.