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目的 研究多药耐药性相关蛋白基因———MRP基因 (multi drugresistanceassociatedproteingene ,MRPgene)对非小细胞肺癌 (non smallcelllungcancer,NSCLC)患者预后的影响。方法 应用地高辛标记探针原位分子杂交结合免疫组化技术检测 47例NSCLC患者根治术后石蜡组织标本中癌细胞MRP基因mRNA表达 ,并对患者进行回顾性随访。结果 47例NSCLC组织标本中癌细胞均有不同程度MRP基因mRNA过度表达 ,其表达水平与患者术后生存期、复发及转移有密切关系 ,与临床病理特征无明显关系。结论 MRP基因与NSCLC患者预后有密切关系 ,检测MRP基因mRNA表达水平可作为预测NSCLC患者预后、确定化疗方案的手段之一。
Objective To investigate the effect of multi-drug resistance associated protein gene (MRPgene) on the prognosis of non small cell lung cancer (NSCLC). Methods Digoxin-labeled probe in situ hybridization and immunohistochemistry were used to detect the mRNA expression of MRP gene in 47 paraffin-embedded specimens of NSCLC patients. The patients were followed up retrospectively. Results All the 47 NSCLC specimens had different levels of mRNA expression of MRP gene. The expression level of MRP gene was closely related to the survival, recurrence and metastasis of the patients. There was no significant correlation between MRP mRNA expression and clinicopathological features. Conclusion MRP gene is closely related to the prognosis of patients with NSCLC. Detecting MRP gene mRNA expression level can be used as one of the means to predict the prognosis of patients with NSCLC and to determine the chemotherapy regimen.