目的利用RAPD技术从分子水平对女贞和日本女贞进行鉴定。方法采用改良CTAB法提取女贞和日本女贞叶片DNA,从S系列20对引物中筛选出能稳定扩增的3对引物,对DNA进行PCR扩增,采用琼脂糖凝胶电泳对扩增产物进行分离。结果女贞与日本女贞在DNA方面有共同特征,也存在显著差异。结论 RAPD分子标记技术显示了女贞和日本女贞之间明显的种间差异,可以用于二者的鉴定,为女贞的进一步研究提供实验依据。 Objective To identify privetum and Japanese privetum at the molecular level by RAPD technique. Methods The DNA of Prunella and Japanese privet were extracted by modified CTAB method. Three pairs of primers were screened out from 20 pairs of primers in S series. The DNA was amplified by PCR. The amplified product was analyzed by agarose gel electrophoresis. For separation. Results Privet and Japanese privet share common features in DNA, there are also significant differences. Conclusion The RAPD technique shows the obvious interspecies difference between Primula and Japanese privet, which can be used for the identification of both. It provides the experimental basis for the further study of Primula.