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通过对传统SDS-PAGE电泳技术中样品前处理及制胶技术的改进,开发出了一种能够清晰鉴别Lox-1与Lox-2同工酶的SDS-PAGE电泳快速检测新技术。利用新改进的方法,在289个CS8000(♀)×FJ307(♂)F2杂交后代中,选拔出Lox-1,-2,-3同工酶完全缺失的个体27株,以及Lox-1,-3和Lox-2,-3缺失中间材料若干株。证明新改进方法可以缩短杂交后代脂氧酶缺失个体筛选进程,提高筛选精度,降低筛选成本。
A new rapid detection technique for SDS-PAGE electrophoresis that can clearly identify Lox-1 and Lox-2 isozymes has been developed by improving the sample pre-treatment and gel-forming techniques in the traditional SDS-PAGE electrophoresis. Using the new improved method, 27 individuals with complete deletion of Lox-1, -2 and -3 isozymes were selected from 289 CS8000 (×) × FJ307 (♂) F2 hybrids and Lox-1, 3 and Lox-2, -3 missing intermediate material several strains. It is proved that the new improved method can shorten the screening process of individual with the lack of lipoxygenase in the offspring of hybrids, improve the screening accuracy and reduce the screening cost.