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为了研究鲤鱼肠道完整吸收小肽与肠道中小肽的关系,用6.25%酶解酪蛋白、酸解酪蛋白、酪蛋白溶液和生理盐水对鲤鱼进行肠道灌注试验(1mL/100g体重),20min后尾静脉采血制备血浆,用高效液相色谱仪分析。结果表明,鲤鱼血浆中肽量的增加与肠道提供的肽种类和数量有关,鲤鱼肠道能够完整地吸收某些小肽进入血液循环。
In order to study the relationship between intestinal intact peptides and small peptides in intestinal tract, carp was subjected to intestinal perfusion test (1mL / 100g body weight) with 6.25% protease, casein, casein solution and saline solution. Blood was collected from the tail vein after 20 min, and analyzed by high performance liquid chromatography. The results showed that the increase of carp plasma peptide amount and the type and quantity of peptides provided by the intestine, carp intestinal intact certain small peptides into the blood circulation.