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钙离子在中枢神经系统的功能调控及许多病理过程中都有重要作用。而大多数经典钙拮抗剂对神经元Ca~(2+)通道不敏感。最近我们研究表明某些四氢异喹啉类化合物,如蝙蝠葛苏林碱对神经元Ca~(2+)通道有相对选择性拮抗作用。为了进一步确定四氢异喹啉类化合物对神经元Ca~(2+)通道的拮抗活性,我们观察粉防已碱、蝙蝠葛碱对突触体~(45)Ca内流的影响.实验方法Sprague-Dawley大鼠,体重200±23g,雌雄兼用。大鼠断头,迅速分离大脑皮层,制备突触体。用酚试剂法测定蛋白含量,突触体反应浓度为0.9g/L。按文献测定~(45)Ca内流。高K~+(65mmol/L)去极引起”快相”~(45)Ca内流反应时间为20s。净~(45)Ca内流量△(Ca~(2+))i=高K~+引起“快相”~(45)Ca内流—基础~(45)Ca内流。
Calcium ion plays an important role in the regulation of the function of the central nervous system and many pathological processes. Most classical calcium antagonists are insensitive to neuronal Ca 2+ channels. Recently, we have shown that certain tetrahydroisoquinoline compounds, such as batsulfuronine, are relatively selective antagonistic to neuronal Ca 2+ channels. In order to further determine the antagonistic activity of tetrahydroisoquinoline compounds on neuronal Ca 2+ channels, we observed the effect of tetracycline and dauricine on synaptosome-45Ca influx. Sprague-Dawley rats, weighing 200 ± 23g, both male and female. The rats were decapitated and the cerebral cortex was rapidly isolated to prepare synaptosomes. The protein content was determined by phenol reagent method and the synaptic body reaction concentration was 0.9 g/L. According to the literature, ~(45)Ca influx was determined. High K + (65 mmol / L) depolarization caused “fast phase” ~ (45) Ca inward reaction time was 20 s. The net ~(45)Ca internal flow △(Ca 2+ )i=high K~+ caused the “fast phase” ~ (45) Ca influx - the basis ~ (45) Ca influx.