论文部分内容阅读
目的:评价三七皂苷的溶血性及免疫佐剂作用。方法:以分光光度法测定三七皂苷对红细胞的溶血百分率;以卵白蛋白(OVA) 100μg、OVA 100μg加氢氧化铝2mg及OVA 100μg加不同剂量三七皂苷(50、100、200μg)分别免疫ICR小鼠,二次免疫(间隔14天)后,用MTT法检测Con A、PWM和PHA诱导脾淋巴细胞增殖反应,ELISA检测血清中的抗OVA抗体效价。结果:三七皂苷浓度为500和250mg/L时红细胞的溶血百分率分别为11.6％和3.6％;OVA加三七皂苷100μg免疫组小鼠Con A、PWM和PHA诱导的脾淋巴细胞增殖反应显著高于OVA对照组(P<0.01);OVA加三七皂苷(50、100、200μg)免疫细小鼠血清中抗OVA抗体效价高于OVA对照组(P<0.01)。结论:三七皂苷具有免疫佐剂活性及较低的溶血性。
Objective: To evaluate the hemolytic activity of panax notoginseng and the effect of adjuvant. Methods: Spectrophotometry was used to determine the hemolysis percentage of notoginsenoside on erythrocytes. OVA was immunized with ovalbumin (OVA) 100 μg, OVA 100 μg plus aluminum hydroxide 2 mg and OVA 100 μg plus different doses of notoginsenoside (50, 100, 200 μg). Mice were used to detect the proliferation of spleen lymphocytes induced by Con A, PWM, and PHA by MTT assay. The anti-OVA antibody titer in serum was detected by ELISA. Results: The percentage of hemolysis of red blood cells was notably 11.6% and 3.6% when notoginsenoside was 500 and 250mg/L, respectively. The proliferation of splenic lymphocytes induced by Con A, PWM and PHA was significantly higher in mice immunized with OVA and notoginsenoside 100 μg. In the OVA control group (P<0.01), the anti-OVA antibody titer in serum of OVA plus pancreatic saponin (50, 100, 200 μg) was higher than that of OVA control group (P<0.01). CONCLUSION: Notoginsenoside has immunoadjuvant activity and low hemolytic activity.