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目的:利用穿梭质粒pAd track CMV,探讨抑癌基因IL-24对宫颈癌细胞株Siha细胞生长和凋亡的影响。方法:将携带目的基因IL-24的穿梭质粒pAd track CMV转染入体外培养的宫颈癌细胞株Siha细胞,用MTT法检测细胞的生长增殖,用流式细胞术测定细胞周期及凋亡率。结果:经MTT法测定,3组Siha细胞OD值的总体差异有统计学意义(P<0.001),两两比较,IL-24组Siha细胞OD值明显低于空质粒组和阴性对照组(P<0.001),空质粒组和阴性对照组Siha细胞OD值则无明显差异(P=0.100);经流式细胞术测定,3组Siha细胞早期凋亡率的总体差异有统计学意义(P<0.05),两两比较,IL-24组Siha细胞早期凋亡率明显高于空质粒组和阴性对照组(P<0.05),空质粒组和阴性对照组Siha细胞的早期凋亡率无明显差异(P>0.05);与空质粒组和阴性对照组Siha细胞相比,IL-24组Siha细胞G0/G1期细胞增多(P<0.05),S期细胞减少(P<0.05),G2/M期细胞减少(P<0.05),空质粒组和阴性对照组则无明显差异(P>0.05)。结论:IL-24对宫颈癌细胞有抑制生长、诱导凋亡的作用。
OBJECTIVE: To investigate the effect of tumor suppressor gene IL-24 on the growth and apoptosis of cervical cancer cell line Siha by shuttle plasmid pAd track CMV. Methods: The shuttle plasmid pAd track CMV carrying the target gene IL-24 was transfected into Siha cells cultured in vitro. The proliferation and proliferation of the cells were detected by MTT assay. The cell cycle and apoptosis rate were determined by flow cytometry. Results: The OD value of Siha cells in three groups was statistically significant (P <0.001) by MTT assay. OD value of Siha cells in IL-24 group was significantly lower than that in empty plasmid group and negative control group <0.001). There was no significant difference in Siha cells OD value between empty plasmid group and negative control group (P = 0.100). The flow cytometry showed that there was significant difference in the early apoptotic rate of Siha cells (P < 0.05). The early apoptotic rates of Siha cells in IL-24 group were significantly higher than those in empty plasmid group and negative control group (P <0.05). There was no significant difference in the early apoptotic rate of Siha cells between empty plasmid group and negative control group (P> 0.05). Compared with Siha cells in empty plasmid group and negative control group, cells in G0 / G1 phase of Siha cells increased (P <0.05), S phase cells decreased (P <0.05) (P <0.05), there was no significant difference between empty plasmid group and negative control group (P> 0.05). Conclusion: IL-24 can inhibit the growth and induce the apoptosis of cervical cancer cells.