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SCR是芸薹属自交不亲和性的雄性决定因子。为研究SCR与SRK之间相互作用的核心区段,通过构建结球甘蓝的包含系列不同长度的SCR的cDNA序列的pGBKT7融合载体,利用酵母双杂交系统检测SCR与SRK之间的相互作用。结果显示,构建的载体均未出现自激活现象,所获得SCR全长与其相对应SRK胞外域具有相互作用,且相互作用核心编码区位于SCR编码基因的第97~186bp处。实验结果还显示,此单倍型的SCR信号肽剪切位点及相邻的几个氨基酸残基对相互作用实验结果有干扰作用。以上结论为包括甘蓝在内的芸薹属自交不亲和机制的研究提供了新的实验依据。
SCR is a male determinant of selfing incompatibility in Brassica. To study the core segment of the interaction between SCR and SRK, the yeast two-hybrid system was used to detect the interaction between SCR and SRK by constructing a pGBKT7 fusion vector containing a series of SCR sequences of different lengths of cabbage. The results showed that none of the constructed vectors showed self-activation, and the total length of the obtained SCR interacted with its corresponding SRK ectodomain. The core coding region of the interaction was located at positions 97-186 bp of the SCR gene. The experimental results also showed that this haplotype SCR signal peptide cleavage site and several adjacent amino acid residues interfere with the experimental results of the interaction. The above conclusions provide a new experimental basis for the research on Brassica self-incompatibility including cabbage.