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用ＳＰ法检测滋养细胞肿瘤、葡萄胎和正常绒毛中ｐ１６蛋白、ＣＤＫ４及ＰＣＮＡ的表达，发现：①ｐ１６蛋白在肿瘤中表达低于绒毛中（Ｐ＜０．０５），ｐ１６蛋白阳性标本的ＰＣＮＡ表达率低于ｐ１６阴性者（Ｐ＜０．０５）。②ＣＤＫ４表达在滋养细胞肿瘤、葡萄胎和绒毛三者间及与ＰＣＮＡ表达间无差异（Ｐ＞０．０５）。③ｐ１６阳性肿瘤患者３年存活率较高。提示ｐ１６基因突变可能是滋养细胞癌变的重要原因。检测ｐ１６蛋白表达有助于估计患者预后。
SP method was used to detect the expression of p16 protein, CDK4 and PCNA in trophoblastic tumors, hydatidiform moles and normal villi. It was found that: 1 p16 protein expression was lower in the tumors than in the villus (P<0.05). PCNA expression was positive in p16 protein samples. The rate was lower than those with negative p16 (P<0.05). 2The expression of CDK4 was not different between trophoblastic tumor, hydatidiform mole and villus and PCNA expression (P>0.05). The 3-year survival rate of 3p16-positive tumors is higher. It suggests that p16 gene mutation may be an important cause of trophoblastic carcinogenesis. Detecting p16 protein expression helps to estimate the patient’s prognosis.