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1989年从哈尔滨市郊区罹病八字地老虎(Xestiac-nigrum)幼虫中分离获得八字地老虎核型多角体病毒(X_(c-n)NPV),国内过去未见报道。多角体多为四方形、三角形或不规则形,大小约1.0～2.5μ。由镜下观察病毒粒子杆状,长230～260nm,宽20～30nm。超薄切片观察表明,该病毒为多粒包埋型,多为3～5粒为一束。对2龄八字地老虎幼虫毒力测定表明,8天内的 LC_(50)(致死中浓度)为3.3×10~5PIB/mL。以3.7×10~7PIB/mL 浓度的病毒感染3龄末期幼虫所获病毒产量最高,可用于病毒增殖。
In 1989, the eight-spot tiger nuclear polyhedrosis virus (X_ (c-n) NPV) was isolated from larva of Xestiac-nigrum, a suburb of Harbin, and it has not been reported in the past. Polyhedron mostly square, triangle or irregular shape, the size of about 1.0 ~ 2.5μ. By microscopic observation of the virus particle rod, length 230 ~ 260nm, width 20 ~ 30nm. Ultra-thin section observation showed that the virus was multi-embedding, mostly 3 to 5 for a bunch. The virulence of the 2-instar tiger larvae showed that LC_ (50) (lethal concentration) within 8 days was 3.3 × 10 ~ 5PIB / mL. The virus produced by the third instar larvae infected with virus at the concentration of 3.7 × 10 ~ 7PIB / mL had the highest virus yield and could be used for virus proliferation.