实际上,外科病理学家每天都会碰到许多用常规光镜检查不足以作出精确病理学诊断的病例。多年来,为了克服这一障碍,建立了许多辅助诊断技术——殊特组织染色、组织化学反应、电子显微镜检查以及新近兴起的免疫荧光和免疫组织化学,乃至更新兴的技术。由于重组 DNA 方面异乎寻常的进展为病理学家提供了另一种诊断手段:即应用 DNA探针对病理材料中特殊核酸序列进行检测来诊断疾 In fact, surgical pathologists encounter many cases each day that are not routinely examined by light microscopy to make an accurate pathological diagnosis. Over the years, in order to overcome this obstacle, many auxiliary diagnostic techniques have been established, such as special tissue staining, histochemical reaction, electron microscopy and the newly emerging immunofluorescence and immunohistochemistry, and even more recent technologies. Due to the extraordinary advances in recombinant DNA, pathologists have also provided another diagnostic tool for the diagnosis of disease by detecting specific nucleic acid sequences in pathological materials using DNA probes