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以芜菁油菜为材料，从无菌苗５—８天叶龄的叶片分离叶肉原生质体，然后以６×１０￣４／ｍＬ的密度用ＭＰ１－３液体培养基进行浅层培养。培养１０天后，用ＭＰ４培养基对ＭＰ１－２中的培养物进行稀释（１：１）．用ＭＰ５培养基对ＭＰ３中的培养物进行稀释（１：１），每次间隔１０天。在培养期间时常用手轻轻摇动培养物。用这种方法，再生细胞分裂并形成愈伤组织，愈伤组织经ＭＳ１培养基增殖３周，转到分化培养基上诱导植株分化，分化出的苗经诱导生根发育成完整的小植株（共５４株）．在研究中发现：①激素的种类、浓度及组合对刺激细胞分裂的作用差异明显；②提高培养基的ｐＨ值可明显地控制细胞褐化；③ＡｇＮＯ＿３（５ｍｇ／ｍＬ）有益于愈伤组织分化植株；④谷氨酰胺（８０ｍｇ／ｍＬ）和腺嘌呤（４０ｍｇ／ｍＬ）能提高愈伤组织的植株分化频率；⑤原生质体培养基对原生质体植株的分化亦有影响．
With the turnip rape as material, the mesophyll protoplasts were isolated from leaves of 5-8 day-old sterile seedlings and then cultured in MP1-3 liquid medium at a density of 6 × 10 ~ 4 / mL. After 10 days of culture, the culture in MP1-2 was diluted with MP4 medium (1: 1). Cultures in MP3 were diluted (1: 1) with MP5 medium at intervals of 10 days. Use gentle shaking of the culture with hands during the cultivation. In this way, regenerated cells divide and form callus. Callus tissue is propagated for 3 weeks on MS1 medium and transferred to differentiation medium to induce plant differentiation. The differentiated shoots induce rooting to develop into complete plantlets 54 strains). In the study, it was found that: (1) The effect of hormone type, concentration and combination on stimulating cell division was obvious; (2) Increasing culture medium pH could obviously control cell browning; (3) AgNO 3 (5mg / mL) ; ④ glutamine (80mg / mL) and adenine (40mg / mL) can increase the frequency of plant differentiation of callus; ⑤ protoplast culture medium also affect the protoplast differentiation.