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目的:发状根生产的次生代谢产物含量比植物自身合成的含量高几倍甚至几十倍,利用人参发状根可以生产大量人参皂苷,并有效的抑制癌细胞的增值。方法:通过正交试验设计筛选人参发状根诱导的条件,通过发状根生物积累量和皂苷含量的测定确定培养条件,然后利用MTT法测定了人参皂苷对HepG2细胞的影响。结果:本实验优化了人参发状根的最佳诱导条件,吸光度A 0.6,侵染时间为10 min,预培养时间为3 d,筛选出发状根的最佳培养条件,MS培养基,pH 6.1,24℃培养时,发状根的生物积累量和皂苷含量均较高,采用人参总皂苷对肝癌细胞HepG2的抑制效果进行研究,证实人参总皂苷对HepG2的抑制率与给药浓度呈正相关。结论:筛选出最适的人参发状根诱导及培养条件,不同部位的总皂苷对肝癌细胞HepG2的增殖均具有抑制作用。
OBJECTIVE: The content of secondary metabolites produced by hairy roots is several times or even several times higher than that of plants. Using ginseng root hairy roots can produce large amounts of ginsenosides and effectively inhibit the proliferation of cancer cells. Methods: The orthogonal test was used to select the conditions for the induction of hairy roots of Ginseng. The culture conditions were determined by measuring the bioaccumulation of hairy roots and the content of saponins. Then the effect of ginsenosides on HepG2 cells was determined by MTT assay. Results: The optimal conditions of hairy roots were optimized with absorbance A 0.6, infection time 10 min and pre-culture time 3 d, and the best culture conditions of root-like roots were screened. MS medium, pH 6.1 , The biological accumulation of hairy roots and the content of saponins were higher at 24 ℃. The inhibitory effect of ginsenosides on HepG2 cells was studied. It was confirmed that the inhibition rate of ginsenoside to HepG2 was positively correlated with the concentration of ginsenoside. Conclusion: The best induction and culture conditions of hairy root of Panax ginseng were screened, and the total saponins of different parts had inhibitory effects on the proliferation of HepG2 cells.