对硫酸庆大霉素Ｃ组分进行了测定，并用中国药典、英国药典、美国药典不同的计算方法，对实验结果进行计算。通过分析实验结果，比较三国药典的计算方法和色谱控制条件，可以看出，美国药典的方法相对优越 Gentamicin sulfate C component was determined, and the Chinese Pharmacopoeia, the United Kingdom Pharmacopoeia, the United States Pharmacopoeia different calculation methods, the experimental results were calculated. By analyzing the experimental results and comparing the calculation methods and chromatographic conditions of Pharmacopoeia of the Three Kingdoms, it can be seen that the method of the U.S. Pharmacopeia is relatively superior