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用硅烷偶联剂与多孔二氧化硅小球反应制备了6种不同化学基团修饰的多孔硅球,研究该界面材料的亲疏水性对蛋白质聚集和结晶行为的影响。通过动态光散射(DLS)和原子力显微镜(AFM)对溶菌酶溶液中聚集体粒径的表征发现:材料的多孔结构能够促进无序多聚体的解聚,防止其阻碍晶体生长;此外,若多孔硅球的界面疏水,则能加快这一过程,使溶液迅速达到只有寡聚体的单分散状态,从而提高结晶速率,亲水界面则相反。扫描电镜(SEM)观察材料界面的晶体形貌进一步表明:具有疏水界面的多孔硅球能更快、更多地诱导蛋白质成核,并通过调控溶液中蛋白质聚集行为来加速晶体生长。
Six kinds of porous silicon balls modified with different chemical groups were prepared by reacting silane coupling agent with porous silica beads to study the effect of hydrophilicity and hydrophobicity of the interface material on aggregation and crystallization behavior. The characterization of aggregate size in lysozyme solution by dynamic light scattering (DLS) and atomic force microscopy (AFM) found that the porous structure of the material can promote disaggregation of disordered multimer to prevent it from hindering crystal growth; in addition, if Hydrophobic interface of porous silica ball can speed up the process, so that the solution can reach the monodisperse state of oligomer only rapidly, so as to increase the crystallization rate, while the hydrophilic interface is the opposite. Scanning electron microscopy (SEM) observation of the crystal morphology at the material interface further demonstrated that porous silicon balls with a hydrophobic interface induced protein nucleation more rapidly and more and accelerated crystal growth by regulating protein aggregation behavior in solution.