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目的对广州市花都区789例疑似人乳头瘤病毒(HPV)感染的妇女进行调查,研究HPV感染状况和基因亚型分布情况。方法采用荧光定量聚合酶链式反应(Fluorescence quantitative PCR,FQ-PCR)对789例疑似HPV感染患者的宫颈分泌物进行21种人乳头瘤病毒基因亚型的检测。结果 (1)789例标本中,HPV阳性占48.42%(382/789),共检出10种基因型,其中检出单一低危型(HPV6、11、43和44型)279例,占感染者73.04%;单一高危型(HPV16、18、33、52、53、58)68例,占感染者17.80%;多重感染35例,占感染者9.16%。低危型以HPV6、11为主,高危型以HPV16、18为主。HPV6为主要致病基因型,检出率为34.0%(P<0.01);(2)各年龄段间感染率差异无统计学意义(P>0.05)。结论花都区HPV感染率较高,且多为单一轻危型感染,以HPV6为主要感染亚型,HPV基因型分布有助于确定宫颈癌发生的高危因素及筛查高危人群,对本地区HPV疫苗开发、应用具有重大意义。
Objective To investigate the prevalence and genotype distribution of HPV in 789 women infected with human papillomavirus (HPV) in Huadu District of Guangzhou City. Methods Twenty-one human papillomavirus genotypes were detected in cervical secretions of 789 patients with suspected HPV infection by fluorescence quantitative polymerase chain reaction (FQ-PCR). Results (1) Of the 789 specimens, HPV positive was 48.42% (382/789). A total of 10 genotypes were detected, of which 279 were single low risk type (HPV6, 11, 43 and 44) 73.04%. A single high-risk type (HPV16,18,33,52,53,58) 68 cases, accounting for 17.80% of infected persons; multiple infections in 35 cases, accounting for 9.16% of infected persons. Low-risk type to HPV6, 11-based, high-risk type to HPV16,18-based. The detection rate of HPV6 was 34.0% (P <0.01). (2) There was no significant difference in the infection rates among all age groups (P> 0.05). Conclusion The prevalence of HPV in Huadu District is high, and most of them are single mild-type infections. HPV6 is the main subtypes of HPV infection. The distribution of HPV genotypes is helpful to determine the risk factors of cervical cancer and to screen high-risk groups. Vaccine development, application of great significance.